λ-DNA labelled with intercolating dye Sytox Orange. The color represents the estimated azimuthal angle of the dye from single molecule fitting using a "Vortex" PSF. The dye orientation is perpendicular to the orientation of the DNA strand. Scale bar 10 μm. Hulleman et al. Nature Communication 2021 Click.

3D reconstruction from 306 individual super-resolution 3D PAINT images of the Nuclear Pore Complex (NPC). Here protein nup107 is fluorescently labeled. You clearly see two rings at about 60 nm distance resolved and the eight fold symmetry of the structure. Animation from our publication Heydarian et al. Nature Communication 2021 Click.

Reconstruction from 383 individual super-resolution 2D PAINT images of DNA-origami structures of the TU Delft logo. We obtain wavelength/175 resolution. Each "blob" shows one binding site for fluorophores on the DNA-origmai structure. These sites are 5 nm apart (twice the width of DNA). Image from our publication Heydarian et al. Nature Methods 2018. Click.

Combination of Structured Illumination and Localization Microscopy ("SIMflux", as combination of SIM and MINflux). By excitation of single emitters with a spatial varying and shifting pattern, we decrease the required number of photons by about twofold compared to standard single molecule localization micorscopy. Image from our publication Cnossen et al. Nature Methods 2020 Click.

A design for a cryogenic fluorescence microscope and the possibility to image fixed single emitters with varying polarized excitation. Cover image from our publication Hulleman et al. Small Methods 2018 Click.

Infographics of TU Delft logo for super-resolution microscopy. It exemplifies one of the main conclusions from our publication Nieuwenhuizen et al. Nature Methods 2013 where we show that localization uncertainty and density of localizations together determine the interpretable resolution in images beyond the diffraction limit. We adpoted a method from electron microscopy Fourier Ring Correlation (FRC). Click.

Overlay of data fusion from fluorescence super-resolution data of several hundred nuclear pore complexes (NPC) and the negatively stained nuclear envelope of a Xenopus laevis oocyte (sub-sahara frog). Fluorophore Alexa647 is immunostained to the nucleoporin (inner ring 41 nm diameter) and gp210 in the nuclear envelope (outer ring with eight-fold symmetry and 164 nm diameter). The image is generated from data of our publication Löschberger et al. Journal of Cell Sciences 2012. Click.